柱前衍生高效液相色谱法测定注射液中白消安的研究

Determination of busulfan injections with pre-column derivatization by high performance liquid chromatography

建立了一种以二乙基二硫代氨基甲酸钠（DEDC）为柱前衍生化试剂测定白消安的反相高效液相色谱分析方法.采用C18色谱柱（250 mm×4.6 mm,5μm）,以V（乙腈）：V（水）：V（四氢呋喃）=55：25：20为流动相,流速为1.0mL/min,检测波长为278 nm.白消安在119.0～892.5 μg/mL范围内呈良好的线性关系,r=0.9988,回收率99.81%～99.89%.

A new sensitive RP-HPLC metiod for the determination of busulfan injections using ultra violet spectrometric detection was developed. The sample reacted with Sodium diethyldithiocarbamate （DEDC） and was analyzed on a C18（250 mm ×4.6 mm, 5 μm） column at room temperature. The mobile phase was composed of acetonitrile-water-tetrahydrofuran （55：25：20）, and the flow rate was 1.0 mL/min. The detection wavelength was 278 nm. Under the chromatographic conditions, a good linear relationship between the peak area and the quantity of busulfan was obtained in the range from 119.0 to 892.5 μg/mL （ r =0.998 8）. The range of recovery with standard addition was from 99.81% to 99.89 %.