激素相关性股骨头坏死股骨头内VEGF和BMP-2的表达

Study of VEGF and BMP-2 genes expressions inside the necrotic rabbit femoral head

[目的]研究兔激素相关性股骨头坏死股骨头内血管内皮生长因子(vascu lar endothelial growth factor,VEGF)和骨形态发生蛋白-2(bone morphogenetic prote in-2,BMP-2)基因表达的动态变化特点。[方法]用LPS(10μg/kg体重)间隔24 h两次静注加MPS 3次肌注的方法制作的兔股骨头坏死模型,设计兔相关的探针和引物,通过免疫组化、原位杂交并直接提出股骨头内总RNA进行实时荧光定量PCR,研究不同时相点兔坏死股骨头内VEGF和BMP-2基因表达的动态变化特点。[结果]在造模至第4、8、12、16周时,股骨头内VEGF和BMP-2基因的表达免疫组化、原位杂交结果均为阴性;实时荧光定量PCR结果发现表达量均远低于空白对照组。[结论]在LPS加MPS制作的兔股骨头坏死模型中,股骨头内的VEGF和BMP-2基因表达均受抑制。

[ Objective] To study the dynamic changes of the VEGF and BMP-2 genes expressions inside the necrotic femoral head of rabbits. [Method] Made rabbit model of femoral head necrosis （FHN） with the method of twice intravenous injection with low dosage lipo polysaeeharide （LPS） （ 10 μg/kg body weight, at a time interval of 24 hours. ） and 3 times intramuscular injection with methylprednisolone （MPS） （ 20mg/kg body weight, at a time interval of 24 hours. ）. ]mmunobistochemistry, in situ hybridization and extract total RNA for real-time quantitative PCR were performed and successfully designed the suitable probes and primerswas used to detect the dynamic changes of the VEGF and BMP-2 genes expressions inside the femoral head of rabbits. [Result] The expressions of the VEGF and BMP-2 genes in group B （model group） at the4, 8, 12 th and 16 th week were negative and real-time quantitative PCR showed that the level of the VEGF and BMP-2 mRNA genes expressions were obviously lower than that of control group. [ Conclusion] The VEGF and BMP-2 genes expressions are inhibited in FNH model induced by LPS and MPS.