胃癌生长抑素受体mRNA表达的研究

Study on mRNA expressions of subtypes of somatostatin receptors in gastric carcinoma

目的:研究胃癌组织中生长抑素受体(SSTR)各亚型的分布与表达特性。方法:用逆转录聚合酶链反应(RT-PCR)检测24例胃癌肿瘤组织与癌旁正常组织中5种SSTR亚型mRNA的表达。结果:胃癌肿瘤组织及癌旁组织中SSTR1、2、5mRNA阳性表达率均为100%(24/24);SSTR3、4mRNA在肿瘤组织的阳性表达率分别为79%(19/24)和71%(17/24),在癌旁组织的阳性表达率分别为83%(20/24)和75%(18/24)。RT-PCR产物凝胶定量分析结果表明胃癌肿瘤组织各型生长抑素受体mRNA的表达量均较癌旁组织为低,但差异无统计学意义,P>0·05。胃癌肿瘤组织中以SSTR2mRNA表达量最高,其次为SSTR5、SSTR1、SSTR3mRNA,SSTR4mRNA表达量最低。结论:胃癌肿瘤组织中存在5种生长抑素受体亚型的表达,并以SSTR2的表达最为显著。

OBJECTIVE： To study the expression and distribution of 5 subtypes of somatostatin receptors （SSTR1-5） in gastric carcinoma. METHODS： The mRNA expressions of SSTR1-5 in central and adjacent tissues of gastric carcinoma were detected by RT-PCR technique, RESULTS：Positive expression rates of SSTR1, SSTR2 and SSTR5 mRNA were 100% in central and adjacent tissues of gastric carcinoma, while SSTR3 and SSTR4 mRNA were 790/00 and 71% in central tissue and 83% and 75% in adjacent tissue of gastric carcinoma, respectively. The mRNA expressions of 5 SSTR in tumor tissue were lower than whose in adjacent tissue, but there was no significant difference between central and adjacent tissues ＇, P 〉 0. 05. CONSLUSION：There is mRNA expressions of 5 subtypes of somatostatin receptors in gastric carcinoma, and the mRNA expression of SSTR2 is higher than that of the others.