颞叶癫痫模型和失神癫痫模型的行为学、组织学对比研究

Comparison of Behavioral and Histological Changes between Pilocarpine-Induced Temporal Epilepsy Model and Pentylenetetrazole Kindling Absence Model in Rats

目的比较匹罗卡品癫痫模型及戊四唑点燃癫痫模型的行为学特征及组织学特征。方法制作两种不同的癫痫模型,应用Vedio观察行为学特征,不同时点Nissl染色及Neotimms'染色,对比研究神经元丢失及苔藓出芽的变化。结果戊四唑点燃模型无持续性癫痫自发发作,癫痫产生过程中无神经元丢失及苔藓出芽现象;匹罗卡品癫痫持续状态模型在海马的CA1、CA3及齿状回的门区出现神经元丢失,在大鼠出现反复自发发作的同时出现苔藓纤维出芽现象。结论戊四唑点燃模型类似人类失神癫痫特征,匹罗卡品癫痫持续状态模型类似人类慢性颞叶癫痫特征,神经元丢失和苔藓纤维出芽可能是癫痫发生的原因,是一理想的颞叶癫痫动物模型。

Objective To investigate and compare the behavioral changes, neuron loss of hippocampus and mossy fiber sprouting between piloearpine-indueed status epilepticus （SE） model and pentylenetetrazole （PTZ） kindling model in rats. Methods After two different epilepsy models were made, Vedio was adopted to observe the behavioral changes. Nissl staining and Neo-timms＇ staining were separately used to observe and compare the neuron loss of hippocampus and mossy fiber sprouting in the dentate gyrus （DG） at different time points during epileptogenisis. Results No recurrent spontaneous seizure, no neuron loss and no mossy fiber sprouting were found in PTZ kindling model; whereas obvious neuron loss was found in CA1, CA3 of hippocampus and hilus of DG, and mossy fiber sprouting were found in pilocarpine model in parallel with recurrent spontaneous seizures. Conclusion PTZ kindling model resembles absence epilepsy in human, while piloearpine-induced status epilepticus model resembles chronic temporal epilepsy in human. Neuron loss and mossy fiber sprouting may play an important role in epileptogenisis. Pilocarpine induced epilepsy model can be regarded as an ideal chronic temporal epilepsy model.