成纤维细胞生长因子1基因启动子多态性与晚发型阿尔茨海默病的相关性研究

Correlation between the Promoter Polymorphism of Fibroblast Growth Factor 1 gene and Late-onset Alzheimer's Disease

目的探讨成纤维细胞生长因子1(FGF-1)基因启动子多态性是否与晚发型阿尔茨海默病(LOAD)相关。方法收集206例尸体检查的样本,包括100例LOAD和年龄匹配的对照组106例。PCRRFLP(Restrictionfragmentlengthpolymorphism)法分析FGF1基因启动子(-1385A/G)的基因型。结果FGF1基因启动子(-1385A/G)的基因型频率分布是:AA型20例(10%),GA型89例(43%),GG型97例(47%),在LOAD组和对照组之间,不同基因型频率分布有显著性差异(P=0.027);GG基因型与LOAD呈正相关(oddsratio=2.02,95%CI:1.16～3.52)。结论FGF1基因启动子(-1385A/G)多态性与LOAD显著相关。

Objective To evaluate the correlation between the promoter polymorphism of Fibroblast Growth Factor 1 （FGF-1） and late-onset Alzheimer＇s disease （LOAD）. Methods Clinic pathological data from 206 autopsies were analyzed, including 100 autopsy-confirmed LOAD patients and 106 age-matched non-demented controls. PCR-RFLP （Restriction fragment length polymorphism） approach was used to determine the genotype of the promoter polymorphism of FGF-1 gene. Results The genotyping frequencies of the promoter polymorphism （-1385 A/G） were AA 20 （10%）, GA 89 （43%）, GG 97 （47%）, respectively. There was significant （P = 0.027） difference of genotyping frequencies between the cases and controls; GG genotype was positively associated with LOAD （odds ratio = 2.02, 95% CI： 1.16～3.52）. Conclusion The promoter polymorphism （-1385 A/G） of FGF-1 gene was associated with LOAD.