种植施万细胞的脱细胞同种异体神经移植物对大鼠坐骨神经缺损的修复作用

The Repairing Effect of Acellular Nerve Allograft Transplanted with Cultured Schwann Cells on the Sciatic Nerve Gap of Rat

目的观察种植施万细胞的脱细胞同种异体神经移植物,桥接大鼠坐骨神经缺损后的神经再生。方法应用酶反复消化法与差速贴壁法体外分离培养乳鼠施万细胞;显微注射法将施万细胞种植到脱细胞同种异体神经移植物内;再应用种植施万细胞的脱细胞同种异体神经移植物桥接大鼠坐骨神经10 mm缺损。光镜、透射电镜和扫描电镜观察再生神经的形态结构、有髓神经纤维数量、平均髓鞘厚度并进行统计学分析。结果光、电镜观察到实验组(SCs+ARSN)的施万细胞在再生神经纤维中互相连结纵行排列成类似Büngner带样细胞链,对照组(ARSN)未见到施万细胞的链状排列。实验组再生有髓神经纤维的髓鞘厚度较对照组均匀且较厚,有髓神经纤维数量和平均髓鞘厚度明显多于对照组(P<0.05)。结论种植施万细胞的脱细胞同种异体神经移植物对缺损的坐骨神经再生有更加有效的促进作用。

Objective To observe the effect of acellular nerve allograft transplanted with cultured ,Schwann cells bridging the sciatic nerve gap of rat. Methods Schwann cells （SCs） were isolated from neonatal rats and enzymafically digested with collagenase and dispase in vitro. SCs were microinjected into 10mm long acellular rat sciatic nerve （ARSN） which bridging a surgically created gap on the rat sciatic nerve. The morphology, number of myelinated fibers and mean thickness of myelin sheath of regenerated nerves were observed by light microscope, transmission electron microscope （TEM） and scanning electron microscope （SEM）. Results Büngner strip like cell-chain array of SCs was observed in regenerated never fibers by TEM and SEM in experimental group, but not observed in control group. There was significant difference in the number of myelinated fibers and mean thickness of myelin sheath of regenerated nerves fibers between ARSN with SCs and ARSN （ P 〈 0.05 ）. Conclusion The acellular nerve allografts transplanted with SCs could significantly promote the regeneration of injured sciatic nerve.