宫颈癌细胞产生的免疫抑制因子对IL－2作用的影响

Effect of Tumor-derived Immunosuppressive Factor from Cervical Caritinoma Cells on IL-2

人宫颈癌细胞产生的免疫抑制因子（ＴＤＳＦ）作用于人外周血单个核细胞（ＰＢＭＣ）６ｈ，即可抑制白细胞介素２（ＩＬ－２）产生，与对照组相比，Ｐ＜０．０１。当ＴＤＳＦ存在时，经ＰＨＡ－Ｐ驯化的ＰＢＭＣ效应细胞对外源性ＩＬ－２反应显著减弱，表明ＴＤＳＦ能抑制ＩＬ－２的作用。ＰＨＡ－Ｐ刺激ＰＢＭＣ增殖，但ＴＤＳＦ使其增殖抑制。表明ＴＤＳＦ能抑制ＩＬ－２产生及其作用；抗癌药对ＴＤＳＦ的分泌有部分阻抑作用。

Cervical cancer cell lines produced a tumor-derived immunosuppressive factor(TDSF),which inhibited the production of IL-2 after incubating with PBMC for 6h.The response of PBMC treated with PHA-P to exogeneous IL-2 was significantly decreased in the presence of TDSF, suggesting that TDSF can inhibit the action of IL2.The effect of PBMC after being stimulated with PHA-P was significantly suppressed by TDSF in does-dependent manner.These findings suggest that the inhibitory action of TDSF on T cell activation and proliferation associated with IL-2.The synthesis and secretion of TDSF in cervical cancer were partially inhibited by anticancer drugs.