特异性iRNA对烧伤患者CD20^＋B细胞及增殖反应的影响

Effect of Specific Immune RNA on the Percentage and the Proliferation Activity of CD20￣+B Cells in Patients with Burn

将２８名烧伤患者随机分成两组，对照组１３例进行常规治疗，治疗组１５例在常规治疗基础上应用特异性免疫核糖核酸（ｉＲＮＡ），同时采用鼠抗人Ｂ细胞ＣＤ２０单克隆抗体（ＭｃＡｂ），借助ＡＰＡＡＰ桥联酶标技术，观察了两组烧伤患者外周血ＣＤ２０＋Ｂ细胞数量（百分比）及增殖反应的变化。结果发现：（１）烧伤后患者外周血ＣＤ２０＋Ｂ细胞数量百分比和ＣＤ２０＋Ｂ细胞对丝裂原刺激的增殖反应均明显低于正常组（Ｐ＜００１）；（２）治疗组经特异性ｉＲＮＡ治疗后ＣＤ２０＋Ｂ细胞数量比治疗前平均增加１１．５％，而对照组患者ＣＤ２０＋Ｂ细胞数量及增殖反应无明显变化，表明特异性ｉＲＮＡ可明显提高烧伤患者外周血ＣＤ２０＋Ｂ细胞数量，并可增强烧伤患者外周血ＣＤ２０＋Ｂ细胞对丝裂原刺激的增殖反应。

patients with burn were randomly divided into two groups.The cofitrol group(n=13)was treated routinely,but for the immuno-therapeutic group(n=15)specific immune RNA(iRNA)was given in addition to the routine treatment.By using monoclonal antibody(McAb)of mouse anti-human B cell CD20 and the APAAP technique,we were able to show that both the percentage and the mitogen-stmulated proliferation of CD20+B cells of peripheral blood were reduced in the burn patients.However,treatment with the specific iRNA could elevate the percentage of CD20+B cells and enhance their proliferation in the immuno-therapeutic group.These results show a promise to use the specific iRNA for treatment of burn patients.