肝细胞癌形成门静脉癌栓与P_53、PCNA表达的关系

Correlations between portal vein tumor thrombogenesis and expression of P53and PCNA hepatocellular carcinoma

作者检测９９例手术切除的肝细胞癌（ＨＣＣ）中Ｒ５３蛋白和ＰＣＮＡ的表达。９９例中合并肉眼下门静脉癌栓（ＴＴＰＶ）３９例，合并镜下ＴＴＰＶ３０例，无ＴＴＰＶ３０例。总体Ｒ５３表达阳性率为２４．２４％，ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）为３４．６±２３．６。在上述三组中，Ｐ５３表达阳性率、ＰＣＮＡＬＩ差异均有显著性，ＰＣＮＡＬＩ与Ｒ５３表达密切相关，Ｐ５３表达阳性的ＨＣＣ，其ＰＣＮＡＬＩ显著高于Ｐ５３表达阴性者。结果提示，Ｐ５３基因突变，ＰＣＮＡ活性增强，ＨＣＣ增殖水平由此而升高，可能是ＨＣＣ细胞获得更强侵袭性的重要机制之一，因而也是ＴＴＰＶ形成的机制之一。

Ninety-nine surgical specimens of hepatocellular carcinoma(HCC)were used to determine the expression of P53 protein and that of proliferating,cell nuclear antigen (PCNA).Among them,39 were accompanied by macroscopic tumor thrombi in the portal vein(TTPV),30 with microscopic TTPV and 30 without.The positive rate of P53 expression in 99 HCC specimens was 24.24%, and the mean PCNA Iabel index (PCNA LI) was 34.6±23.6 .The difference of positive rates of P53 expression as well as the PCNA LI in three groups of HCC is significant statistically. There was a strong correlation between P53 expression and PCNA LI. PCNA LI was higher in group with positive expression of P53 than that with negative expression. The results implicate that P53 mutation,which may induce increase of PCNA activity and result in higher proliferative and invasive potentials, is one of the mechanisms of the genesis of TTPV.