硒缺乏大鼠胰岛损害的免疫组织化学图像分析

IMMUNOHISTOCHEMICAL QUANTIITATIVE IMAGE ANALYSIS OF ISLETS LESLIONS OF RATS IN SELENIUM DEFICIENCY

应用免疫组织化学与图像分析技术相结合的方法，较系统地研究了硒缺乏大鼠胰岛的损害。结果表明，晒缺乏可引起胰岛多项结构参数异常，其主要改变为胰岛素免疫反应阳性物面积与胰腺组织面积百分比（Ｉｎｓ＋／ｐａｎｃ）明显下降；胰岛素免疫反应阳性物与胰岛组织面积百分比（Ｉｎｓ＋／Ｉｓｌ）亦显著降低；胰岛平均截面积（Ｉｓｌａｒｅａ）及胰岛素阳性物平均面积（Ｉｎｓ＋ａｒｅａ）不同程度地减少；胰岛形状因子（ＳＦ）增高；胰岛截面数密度（ＮＡ）变化不明显。在胰岛上述结构参数改变的同时，出现血清胰岛素水平降低，胰岛素分泌贮备减少。提示硒缺乏引起胰岛损害的主要变化是以Ｂ细胞为主体的结构与功能异常。

mmunohistochemical and quantitative image analysis methods were usedto investigate the islets lesions of selenium deficient rats.The results de一monstrated that there were morphological lesions in the selenium deficientrats,The main changes were: the ratio of insulin immune active productarea to pancreatic tissue area as well as that to islets area obviously redu-ced;average islet area and insulin immune active product area also dimini-shed;the shape factor of islets increased.The above structural changes of islet lesions were accompanied withthe decrease of insulin concentrations not only in seruin but also in islets tissues.The study suggcsted that selenium deficiency can cause both the func-tional and structural lesions of islets.