肺癌组织中表皮生长因子受体的研究

EPIDERMAL GROWTH FACTOR RECEPTOR IN PRIMARY HUMAN LUNG CANCER

用１２５Ｉ标记的表皮生长因子（ＥＧＦ）为配体测定１２例人肺癌细胞膜表皮生长因子受体（ＥＧＦＲ）。肺癌组织及对照组织细胞膜高亲和力位点的解离常数分别为１．４８和１．１ｎｍｏｌ，肺癌组织ＥＧＦＲ为２．２５～１９．３６ｐｍｏｌ·ｇ－１膜蛋白，高于对照组织的０．７２～７．４３ｐｍｏｌ·ｇ－１膜蛋白，说明ＥＧＦＲ在肺癌演变中起重要的动力作用。

Cell membranes were prepared from 12 human lung cancers for the study of the expression of epidermal growth factor receptors (EGFR). EGFR concentration was estimated by ligand binding studies using 125 I-radiolabeled EGF. The dissociation constants of the high affinity sites were identical, 1. 48nmol and 1. 1nmol in cancer and normal lung tissues,the EGFR contents were higher in lung cancer tissues (range: 2. 25to 19. 36pmol. g-1 membrane protein)than that in normal tissues from the same patients (range: 0. 72 to 7. 43pmol · g-1 membrane protein). These results suggest that EGF and its receptor may play a role in the regulatory mechanisms in the control of lung cellular growth and tumor promotion.