缺血缺氧后新生大鼠海马NR1-mRNA表达的变化及羟丁酸钠对其的影响

Effects of Sodium hydroxybutyrate on expression of hippocampal N-methyl-D-aspartate receptor 1 subunit mRNA in neonatal rat with hypoxic-ischemic insult

Objective To investigate the effect of sodium hydroxybutyrate(GHB) on expression of hippocampal N-methyl-D-aspartate receptor 1 subunit (NR1) mRNA in the neonatal rat with hypoxic-ischemic insult. Methods One hundred eighty seven-day(7d) old Sprague Dawley rat pups of either sex weighting 10 g～14 g were randomly assigned to sham group(S), normal saline group(C),GHB 50 mg/kg group(γ50),GHB 100 mg/kg group(γ100)and GHB 200 mg/kg group(γ200),n=6.The procedure for the induction of hypoxia-ischemia was done according to the way described by Rice:under ether anesthesia,pups were subjected to left carotid artery ligated followed by 2 h of hypoxic exposure(O2:N2=8﹪:92﹪).The pups in S group were only aparted left carotid artery which was not obturated and no hypoxia.GHB 50 mg/kg, 100 mg/kg, 200 mg/kg was administered i.p immediately after hypoxia,then a time every 8h inγ50、γ100 and γ200 group respectively.The same volume normal saline was administered i.p in the same way in S and C group.Reverse transcriptase polymerase chain reaction(RT-PCR) was used to analyze the expression of NR1 mRNA semi-quantificationally in the left hippocampus at 2 h、6 h、12 h、1 d、3 d、7 d after insult in S、C、γ50、γ100 and γ200 group. Results The difference of expression of NR1 mRNA between S and C group wasn’t significant.At any time after insult, The differences of expression of NR1 mRNA among C and three GHB group were not significant too. Conclusion Hypoxia-ischemia and GHB didn’t significantly affect the expression of NR1 mRNA in neonatal rat hippocampus.

Objective To investigate the effect of sodium hydroxybutyrate （GHB） on expression of hippocampal N-methyl-D-aspartate receptor 1 subunit （NR1） mRNA in the neonatal rat with hypoxic-ischemic insult. Methods One hundred eighty seven-day（Td） old Sprague Dawley rat pups of either sex weighting 10 g - 14 g were randomly assigned to sham group（S）, normal saline group（C） ,GHB 50 mg/kg group （γ50）, GHB 100 mg/kg group （ γ100 ） and GHB 200 mg/kg group （ γ200 ）, n = 6. The procedure for the induction of hypoxia - ischemia was done according to the way described by Rice ：under ether anesthesia,pups were subjected to left carotid artery ligated followed by 2 h of hypoxic exposure（O2 ：N2 =8 % ：92 % ）. The pups in S group were only aparted left carotid artery which was not obturated and no hypoxia. GHB 50 mg/ kg, 100 mg/kg, 200 mg/kg was administered i. p immediately after hypoxia,then a time every 8h inγ50,γ100and γ200 group respectively. The same volume normal saline was administered i. p in the same way in S and C group. Reverse transcriptase polymerase chain reaction （RT- PCR） was used to analyze the expression of NR1 mRNA semi - quantificationally in the left hippocampus at 2 h,6 h,12 h,1 d,3 d,7 d after insult in S,C,γ50,γ100 and γ250 group. Results The difference of expression of NR1 mRNA between S and C group wasn＇t significant. At any time after insult, The differences of expression of NR1 mRNA among C and three GlIB group were not significant too. Conclusion Hypoxia - ischemia and GHB didn＇t significantly affect the expression of NR1 mRNA in neonatal rat hippocampus.