高效液相色谱荧光光谱法测定虎杖中的白藜芦醇及白藜芦醇苷含量

Quantitative determination of resveratrol in the roots of Polygonum Cuspidatum by HPLC and elementary study on their fluorescence

目的：建立高效液相色谱（HPLC）法，分离和测定虎杖中的白藜芦醇的含量。方法：采用NUCLEODUR100—5C18柱（250mm×4．6mm，5μm）；流动相：异丙醇和水二元梯度洗脱系统；流速：0．6mL·min^-1；荧光检测器：激发波长：334m；发射波长：404nm。结果：在选定色谱条件下其白藜芦醇苷和白藜芦醇的线性范围良好，样品加样回收率在96．5％～101．4％，相对标准偏差在0．75％～1．42％。结论：本方法精密度好，结果可靠，适合虎杖中白藜芦苷和白藜芦醇的定量分析。

OBJECTIVE The establish an optimal HPLC method to determine the content of resveratrol from the roots of Polygonum Cuspidaturn. METHODS The content of this compound in the roots of Polygonum Cuspidatum was determined by reversed-phase high liquid chromatography. NUCLEODUR 100-5 C18 column（250 mm × 4. 6 mm, 5 μm）, and a mobile phase program of gradient elution with iso-propyl alcohol and water at a flow rate of 0. 6 mL·min^-1 was employed and fluorescence detection of： λex ： 334 nm,λem： 404 nm RESULTS The good linear relationships were obtained under the optimum conditions for each component. The recoveries of the standards added were 96. 5%- 101.4% while the relatively standard deviations were within the range of 0. 75 % to 1.42 %. CONCLUSION This method is accurate and reliable for the quantities analysis of piceid and resveratrol in the Roots of Polygonum Cuspidatum.