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CCK-8法检测疟原虫HGXPRT蛋白刺激对淋巴细胞的增殖作用 被引量：6

Detection of lymphocyte proliferation stimulated by HGXPRT protein of Plasmodium falciparum
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摘要 目的检测疟原虫HGXPRT蛋白刺激淋巴细胞的增殖效果。方法以HGXPRT蛋白免疫小鼠后制备淋巴细胞,与相应刺激物共同培养后采用CCK-8法检测淋巴细胞的增殖情况。结果不同浓度的HGXPRT对淋巴细胞的刺激效果不同,以5μg/ml时刺激增殖效果最好。结论HGXPRT对淋巴细胞具有增殖作用。 Objective To determine the effect of proliferation of lymphocytes stimulated by Plasmodium falciparum HGXPRT protein. Methods Lymphocytes from BALB/c mice immunized with Plasmodiumfalciparum HGXPRT protein were prepared and the proliferation of lymphocytes was determined by CCK - 8 after jointly cultured with corresponding stimulators. Results Effects of stimulation depended on the concentration of Plasmodium falciparum HGXPRT and the concentration of 5μg/ml was the most efficaeious for the proliferation of lymphocytes. Conclusion Plasmodium falciparum HGXPRT can stimulate the proliferation of lymphocytes.
作者 王冰 张冬梅 潘卫庆
机构地区 第二军医大学病原生物教研室
出处 《中国热带医学》 CAS 2006年第6期949-949,957,共2页 China Tropical Medicine
关键词 HGXPRT蛋白 淋巴细胞增殖 CCK-8 疟原虫 HGXPRT protein Lymphocyte proliferation Plasmodium falciparum
分类号 R382.31 [医药卫生—医学寄生虫学]
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参考文献5

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二级引证文献34

中国热带医学
2006年 第6期

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