饮食和运动干预对2型糖尿病大鼠骨骼肌Cu/ZnSOD mRNA表达的影响

Effects of diet and exercise training on Cu/ZnSOD gene expression in skeletal muscle of type 2 diabetic rats

目的:探讨饮食和运动对糖尿病大鼠骨骼肌细胞Cu/ZnSODmRNA表达及其血浆酶活性的影响。方法:雄性Sprague-Dawley大鼠24只采用高糖高脂饮食后腹腔注射小剂量链脲霉素,建立糖尿病模型,随机分为4组:糖尿病高脂饲料非运动组(DFN,n=6),糖尿病常规饲料非运动组(DRN,n=6),糖尿病常规饲料低强度运动组(DRL,n=6),糖尿病常规饲料高强度运动组(DRH,n=6)。活动平板进行耐力训练8周,测定骨骼肌Cu/ZnSODmRNA和血浆Cu/ZnSOD活性的变化。结果:两运动组骨骼肌Cu/ZnSODmRNA表达显著高于DFN组和DRN组,两运动组之间无显著差异。两运动组血浆Cu/ZnSOD活性显著高于DFN组和DRN组,DRN组血浆Cu/ZnSOD活性显著高于DFN组。结论:饮食调整加运动训练可以促进糖尿病大鼠骨骼肌Cu/ZnSODmRNA表达,提高Cu/ZnSOD酶活性,而运动强度对此无显著影响。

Objective：To explore the effects of diet and exercise training on gene expression of skeletal muscle and plasma activities in Cu/ZnSOD in type 2 diabetic rats.Method：Twenty-four male Sprague-Dawley rats were fed on high sucrose diet with high fat for 4 weeks and then injected with low dose of streptozotocin to establish type the 2 diabetic model. The diabetic rats were divided randomly into four groups： high sucrose and fat diet without exercise （DFN）,regnlar diet without exercise （DRN）,regnlar diet with 75%VO2 max exercise （DRH）. The animals with 55%VO2 max exercise （DRL）. The animals with exercise training were forced to running on motorizod treadmill for 8 weeks. The mRNA expression of skeletal muscle and plasma activity in Cu/ZnSOD were measured.Result：The plasma Cu/ZnSOD activities in the two exercise training groups were significantly higher than that in the DFN and DRN groups,in which plasma Cu/ZnSOD activities in DRN group were remarkably higher than that in DFN group. The expression of Cu/ZnSOD mRNA in skeletal muscle increased significantly in the two exercise training groups,in which there were no significant differences between the two exercise training groups. Condusion：Diet plus exercise training can enhance mRNA expression of skeletal muscle and plasma activity in Cu/ZnSOD which is not associated with the intensity of exercise training.