HPLC法测定头孢羟氨苄甲氧苄啶片中头孢羟氨苄和甲氧苄啶的含量

Simultaneous Determination Content of Cefadroxil and Trimethoprim in Cefadroxil and Trimethoprim Tablets by HPLC

目的:建立一种头孢羟氨苄甲氧苄啶片中头孢羟氨苄和甲氧苄啶的含量测定方法。方法:采用ODS色谱柱(150mm×4.6mm,5μm),以0.01mol/L氯化铵溶液甲醇冰醋酸(741∶258∶1)为流动相,检测波长为230nm,柱温为室温。结果:本方法头孢羟氨苄与甲氧苄啶可充分分离,头孢羟氨苄和甲氧苄啶线性范围分别为12.5～150μg/ml(r=0.9999)和2.5～30μg/ml(r=0.9998),头孢羟氨苄含量回收率为99.44%～100.59%,RSD小于1%;甲氧苄啶含量回收率为98.15%～100.34%,RSD小于2%。结论:本方法简便、灵敏、准确,可同时测定头孢羟氨苄和甲氧苄啶的含量。

Objective： To establish an HPLC for the simultaneous determination content of cefadroxil and trimethoprim tablets. Methods：The chromatographic column was O DS （150 mm × 4.6 mm ,5 μm）with room temperature, the mobile phase was composed of 0.01 mol/L ammonium chloride-methanol-actic acid（741： 258：1 ）with a detection wavelength at 230 nm. Resuh： Cefadroxil and trimethoprim was completely separated. Good linear relations were achieved when the concentration ranges of 12.5 - 150 μg/ml （ r = 0.999 9 ） and 2.5 - 30 μg/ml （ r =-0.999 8 ）, RSD were 99.44% - 100.59% and 98.15% - 100.34%. Conclusion：This metheod is simple, sensitive and accurate, may be used for the quality control of cefadroxil and trimethoprim tablets.