食管鳞癌细胞系EC-109中缺氧诱导因子-1α的表达意义

Expression and significance of hypoxia-inducible factor-1 alpha in esophageal squamous cancer cell line EC-109

目的:探讨缺氧诱导因子-1α(HIF-1α)在食管鳞癌细胞系EC-109中的表达及其意义.方法:采用逆转录聚合酶链反应(RT-PCR)和Westernblot分别检测HIF-1αmRNA和蛋白在常氧及缺氧状态下在食管鳞癌EC-109细胞中的表达.结果:HIF-1αmRNA在缺氧食管鳞癌EC-109细胞中过表达(P<0.05),在缺氧24h时HIF-1αmRNA表达最高,其后随时间延长表达下降;HIF-1α蛋白水平在缺氧食管鳞癌EC-109细胞中无明显上调(P>0.05).结论:低氧可诱导食管鳞癌EC-109细胞中HIF-1αmRNA的过度表达,缺氧状态下HIF-1α蛋白水平较常氧时无明显增加.

AIM： To investigate expression and significance of hypoxia inducible factor-1α （HIF-1α） in human esophageal squamous cancer cell line EC-109. METHODS： Semiquantity reverse transcriptionpolymerase chain reaction （RT-PCR） and Western blot were performed to detect the expression of HIF-1α mRNA and protein in esophageal squamous cancer cell line EC-109 exposed to normal oxygen or hypoxia condition. RESULTS： HIF-1α mRNA was over-expressed in esophageal squamous cancer cell line EC-109 under hypoxia condition （P 〈 0.05）, and reached the peak at 24 h. However, HIF-1α protein had no significant up-regulation in hypoxia （P 〉 0.05）. CONCLUSION： Hypoxia can induce high expression of HIF-1α at mRNA level, but not at protein level .