中药肠炎清治疗小鼠葡聚糖硫酸钠所致结肠炎的机制

Therapeutic mechanism of Changyanqing in treatment of colitis induced by dextran sulfate sodium

目的:探讨肠炎清的疗效及其机制.方法:以葡聚糖硫酸钠(DSS)饮水法复制小鼠实验性结肠炎40只,随机平均分为4组:肠炎清中药组、柳氮磺胺吡啶(SASP)西药组、肠炎清和SASP中西药结合组和模型组.观察肠炎清(灌胃剂量为0.2mL/(20g?d)、1次/d、疗程7d)对疾病活动指数(DAI)和肠组织髓过氧化物酶(MPO)活性及TNF-α、IL-1β和IL-6mRNA表达的影响.结果:与模型组相比,肠炎清可降低DAI(1.413±0.835vs2.167±0.911,P<0.05)和MPO活性(72.4±0.590nkat/gvs117.0±0.902nkat/g,P<0.05),并降低肠组织TNF-α(0.841±0.190vs1.320±0.282,P<0.05)、IL-1β(0.641±0.095vs0.920±0.082,P<0.05)和IL-6mRNA(1.241±0.247vs1.620±0.312,P<0.05)的表达,中西医结合组以上指标下降更为明显(DAI:0.608±0.449;MPO:27.3±0.211;TNF-α:0.339±0.081;IL-1β:0.239±0.073;IL-6:0.639±0.141)(P<0.01).肠炎清与柳氮磺胺吡啶(SASP)的作用相当(P>0.05).结论:肠炎清可治疗DSS结肠炎,其降低肠组织TNF-α、IL-1β和IL-6mRNA的表达可能是其疗效机制之一.

AIM： To investigate the therapeutic effects of Changyanqing on dextran sulfate sodium（DSS）- induced colitis and its mechanism. METHODS： DSS was used to establish the model of experimental colitis in 40 mice. Then the mice were averagely randomized into 4 groups （A, B, C, and D）. The mice in group A, B, and C were treated with Changyanqing, salicylazosulfapyridine （SASP）, and Changyanqing ± SASP, respectively, and those in group D served as controls. Changyanqing was administratered by gastric irragation at a dose of 0.2 mL/（20g·d） for 7 d. The： disease activity index （DAI） and the activity of intestinal myeloperoxidase （MPO） were observed. The levels of tumor necrosis factor-α （TNF-α interleukin-1β （IL-1β） and IL-6 mRNA were detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS： In comparison with those in the controls, the DAI （1.413 ± 0.835 vs 2.167 ± 0.911, P 〈 0.05）, and activity of MPO （72.4 ± 0.590 nkat/g vs 117.0 ± 0.902 nkat/g, P 〈 0.05）, and the expression of TNF-α （0.841 ± 0.190 vs 1.320 ± 0.282, P 〈 0.05）, IL-1β （0.641 ± 0.095 vs 0.920 vs 0.082, P 〈 0.05）, and IL-6 （1.241 ± 0.247 vs 1.620 ± 0.312, P 〈 0.05） mRNA were significantly decreased in group A. The above decreases were more significant in group C （DAI： 0.608 ± 0.449; MPO： 27.3 ± 0.211; TNF-α 0.339 ± 0.081; IL-1β： 0.239 ± 0.073; IL-6：0.639±0.141）（P 〈 0.01）. There was marked difference between group A and B （P 〉 0.05）. CONCLUSION： Changyanqing is effective in the treatment of DSS-induced colitis, which may be associated with the decrease of DAI, MPO activity, and TNF-α, IL-1β and IL-6 mRNA expression.