胃癌及癌前病变中P27和Cyclin E蛋白的表达意义

Expression and significances of P27 and Cyclin E protein in gastric carcinoma and precancerous lesion

目的:探讨P27和CyclinE蛋白在胃癌及癌前病变中的表达及其与胃癌病理参数之间的关系.方法:用免疫组化技术(SP法)对正常胃黏膜(normalgastricmucosa,NGM)、慢性浅表性胃炎(chronicsuperficialgastritisCSG)、慢性萎缩性胃炎(chronicatrophiagastritis,CAG)伴肠上皮化生、慢性萎缩性胃炎伴非典型性增生各20例和胃癌(gastriccarcinoma,GC)60例标本进行免疫组织化学染色,分析P27和CyclinE蛋白表达及其与胃癌临床和病理的关系.结果:各组胃组织中P27和CyclinE蛋白表达阳性率分别为NGM组100%和5%,CSG组85%和10%,CAG伴肠化组70%和20%,CAG伴不典型增生组45%和30%,胃癌组38.3%和40%.胃癌组和CAG伴不典型增生组P27阳性率显著低于其他组(P<0.05),CyclinE阳性率则显著高于其他组(P<0.05).P27和CyclinE在胃癌中的表达分别与肿瘤分化程度、浸润深度及肿瘤临床分期相关,P27蛋白的表达尚与有无淋巴结转移相关.P27和CyclinE蛋白在胃癌中的表达呈显著负相关(r=-0.768,P<0.05).结论:检测胃癌组织中P27和CyclinE蛋白的表达有助于判断肿瘤的进展程度,两者联合检测有助于判断肿瘤预后.

AIM： To-investigate the expression of P27 and Cyclin E protein in gastric carcinoma （GC） and precancerous lesion and their correlations with the clinicopathological parameters. METHODS： Immunohistochemical staining was used to evaluate the expression of P27 and Cyclin E protein in the samples of normal gastric mucosa （NGM, n= 20）, chronic superficial gastritis （CSG, n = 20）, chronic atrophic gastritis （CAG） with metaplasia （n = 20）, CAG with dysplasia （n = 20） and GC （n =60）, and their correlations with the clinicopathological parameters were analyzed. RESULTS： The positive rates of P27 and Cyclin E protein expression were 100% and 5% in NGM, 85% and 10% in CSG, 70% and 20% in CAG with metaplasia, 45% and 30% in CAG with dysplasia, and 38.3%（23/60） and 40%（24/60） in GC,respectively. The positive rate of P27 expression in GC and CAG with dysplasia were lower than that in NGM, CSG, and CAG with metaplasia （P 〈 0.05）, and the positive rate of Cyclin E expression in GC and CAG with dysplasia were higher than that in NGM, CSG, and CAG with metaplasia （P 〈 0.05）. The expression of P27 and Cyclin E were significantly correlated with tumor differentiation, infiltration and clinical stages, and P27 expression was also correlated with lymph nodes metastasis. Furthermore, the expression of P27 was inversely correlated with Cyclin E expression in GC （r = -0.768, P 〈 0.05）. CONCLUSION： it is helpful to detect of P27 and Cyclin E protein expression in the judgment of GC progression, and combined analysis of them is of added prognostic value.