延边地区朝、汉族慢性乙型肝炎患者血清标志物与HBV DNA定量的关系

Relationship between hepatitis B virus serum markers and hepatitis B virus DNA quantities in Korean and Han patients with chronic hepatitis B in Yanbian area

目的:探讨延边地区朝、汉族慢性乙型肝炎患者血清HBVM表现模式与HBVDNA含量的关系.方法:采用荧光定量聚合酶联反应(FQ-PCR)和酶联免疫吸附试验(ELISA)检测慢性乙肝患者1773例(朝鲜族1074例,汉族699例)血清HBVDNA含量及HBVM,比较分析朝、汉族HBVM表现模式与HBVDNA含量的关系.结果:HBVM表现模式中朝、汉族在A组(HBsAg+、HBeAg+、HBcAb+)各占40.69%、47.07%(P<0.01),B组(HBsAg+、HBeAb+、HBcAb+)和C组(HBsAg+、HBcAb+)各占47.07%,38.34%及7.18%,4.58%(均P<0.05).两个民族A、B组HBVDNA的阳性率(A:93.82%,93.92%;B:47.54%,47.39%)相似,在A组HBVDNA含量主要以高含量(≥1014-1016copies/L,70.73%,72.17%)为主,B组多数以低含量(≥106-1010copies/L,51.64%,51.18%)为主.结论:延边地区朝、汉族HBVM表现模式有明显差异,但HBVDNA的阳性率相似,HBVDNA含量与HBVM表现模式明显相关.

AIM： To investigate the relationship between the HBV markers （HBV M） and contents of HBV DNA in Korean and Han patients with chronic hepatitis B （CHB） in Yanbian area. METHODS： The contents of HBV DNA and the levels of HBV M were detected in 1773 patients （Korean, n ： 1074; Han, n = 699） with CHB by fluorescence quantitative polymerase chain reaction （FQ-PCR） and enzyme linked immunosorbent assay （ELISA）, respectively. The data were comparatively analyzed between the two ethnic groups. RESULTS： For HBV M, Korean and Han patients covered percentages of 40.69% and 47.07% （P 〈 0.01）, respectively, in group A （HBsAg＋, HBeAg＋, HBcAb＋）, 47.07% and 38.34% （P 〈 0.05）, respectively, in group B （HBsAg＋, HBeAb＋, HBcAb＋）, and 7.18% and 4.58% （P 〈 0.05）, repectively, in group C （HBsAg＋, HBcAb＋）. The positive rates of HBV DNA had no significant difference between Korean and Han patients （A： 93.82%, 93.92%; B： 47.54%, 47.39%）. The higher content of HBV DNA （≥ 10^14-10^16 copies/L） was prominent in group A （70.73%, 72.17%）, and the lower content （≥ 10^6-10^10 copies/L） was domi- nant in group B （51.64%, 51.18%）in both Horean and Han patients. CONCLUSION： HBV M levels are significantly different between Korean and Han patients with CHB in Yanbian area. The positive rate of HBV DNA is similar, but the content of HBV DNA is correlated with HBV M levels in two ethnic groups.