肝郁脾虚型肠易激综合征与5-羟色胺转运体基因多态性的关系

Association between irritable bowel syndrome of hepatic stagnation and spleen deficiency type and serotonin transporter gene polymorphism

[目的]探讨肝郁脾虚型肠易激综合征(IBS)与5-羟色胺转运体(SERT)基因多态性的关系。[方法]用多聚酶链式反应技术(PCR)对50例肝郁脾虚型IBS患者与96例健康对照者SERT基因的启动子区(5-HTTLP-ER)和内含子2可变数目串联重复序列(VNTRs)多态性进行研究。[结果]肝郁脾虚型组5-HTTLPR基因频率分布是S/S 40.0%,L/S 54.0%,L/L 11.1%。健康对照组的分布频率是S/S 57.3%,L/S 35.4%,L/L 7.3%。2组之间比较差异无统计学意义(P>0.05),但肝郁脾虚型组的L/S基因频率比对照组明显升高(P<0.05)。2组VN-TRs区的多态性比较差异无统计学意义(P>0.05)。[结论]拥有L/S基因型可能是肝郁脾虚型IBS的多个易患因素之一。

[Objective] To investigate the relationship between irritable bowel syndrome （IBS） of hepatic stagnation and spleen deficiency type and serotonin transporter（SERT） gene polymorphism. [Methods] Polymerase chain reaction-base technique （PCR） was used to detect the SERT gene-linked region （5-HTTLPER） and the variable number tandem repeats （VNTRs） in intron 2 in 96 healthy subjects and 50 patients with 1BS of hepatic stagnation and spleen deficiency type. [Results] The 5-HTTLPER genotypes frequencies showed that S/S was 40. 0%, I./S was 54. 0 and L/L was 11.1% in the patients, and S/S 57. 3 %, L/S 35.4 %, L/L 7. 3% in the healthy control. There was no significant difference between two groups （P〈0. 05）. The L/S genotype frequency of 5-HTTI.PER alleles was significantly higher in patient group than that in the controis（P〈0.05）. No significant difference for genotype distribution or the allele frequency of VNTRs was revealed between controls and patient groups（P〉0.05）, [Conclusion] People with L/S genotype had an increase risk of developing IBS of hepatic stagnation spleen deficiency type.