HPLC测定川木通中齐墩果酸的含量

Determination of oleanolic acid in Caulis clematidis armandii by HPLC

目的建立测定水解后的川木通药材中齐墩果酸含量的方法。方法采用HPIE法。Alltima C18色谱柱（250mm×4．6mm，5μm）；流动相为甲醇-水-冰醋酸-三乙胺（89：11：0．04：0．02）；检测波长207nm；流速1．0ml·min^-1；柱温45℃。结果齐墩果酸色谱峰分离度良好，线性范围为0．1003—8．024μg（r=0．9999，n=5），方法的检测限和定量限分别为0．3μg·ml^-1和1．0μg·ml^-1。平均回收率为96．1％，RSD=2.8％（n=5）。结论所用结果准确，重复性好，专属性强，可为川木通药材质量控制提供科学的依据。

OBJECTIVE To develop an HPLC method for the content determination of oleanolic acid in Caulis clematidis armandii after hydrulysis.METHODS In the system of HPLC, a AUtima C18（250 mm × 4.6 mm,5 μm） column was used with a mixture of methanol - water - acetic acid - triethylamine （89：11：0.04：0.02） as the mobile phase. The measuring wavelength was set at 207 rim. The flow rate was 1.0 ml· min ^-1 and the column temperature maintained at 45 ℃. RESULTS The oleanolic acid peak in chromatograms was completely separated from other peaks. The linear range of oleanolie acid was 0. 1003 - 8.024 μg （ r = 0. 9999, n = 5）. The detection limit and quanfitation limit of this method were 0.3μg· ml^-1 and 1.0μg· ml^-1 respectively. The average recovery of oleanolic acid was 96.1% （ RSD = 2.8 %, n = 5）. CONCLUSION The method is accurate, precise and reproducible. It can be used to evaluate the quality of Caulis clematidis armandii.