HPLC测定2,6-二叔丁基对甲酚的含量及有关物质

Determination of butylated hydroxytoluene and its related substances by HPLC

目的建立测定2,6-二叔丁基对甲酚含量及有关物质的方法。方法采用Diamonsil^TM C18 ODS柱（250mm×4．6mm，5μm）；流动相为乙腈-乙醇（65：35），流速0．6ml·min^-1，紫外检测波长278nm，柱温30％。结果在本色谱条件下，2，6-二叔丁基对甲酚与有关物质及溶剂峰分离度符合要求，在300—700μg·ml^-1范围内线性良好（r=0．9998）。结论测定方法简便、准确、灵敏度高，方法可靠，可作为质量控制方法。

OBJECTIVE To establish an RP- HPLC method for the determination of butylated hydroxytoluene and related substances.METHODS The separation was performed with a DiamonsilTM C18 ODS columon（250 min× 4.6 mm,5μm） at 30℃ ,and the mobile phase consisted of acetonitrile,alcohol （65：35）,with the flow rate of 0.6 ml·min^-1 and UV detection wavelength at 278 nm.RESULTS Butylated Hydroxytoluene had a good linear relation over the range of 300 - 700μg· ml^-1 （ r = 0. 9997）. CONCLUSION This method is simple, accurate, sensitive, and applicable for quality control of this preparation.