CD_4^+CD_(25)^+调节性T细胞在免疫性血小板减少性紫癜中的调节作用

Role of CD_4^+CD_(25)^+ regulatory T cell in immune thrombocytopenic purpura

目的检测免疫性血小板减少性紫癜(ITP)患者治疗前后CD4+CD2+5调节性T细胞(Treg)的比例以及变化规律,探讨Treg在ITP发病机制中的作用。方法收集42例ITP患者治疗前、后外周血标本,分离血单个核细胞,采用流式细胞术检测ITP患者治疗前后Treg的比例以及变化。结果ITP患者治疗前Treg比例明显低于正常对照组(P<0.01),治疗后Treg比例显著升高,明显高于治疗前,但仍低于正常对照组(P<0.01);治疗有效组ITP患者Treg比例显著高于治疗无效组(P<0.01),治疗显效组显著高于良效和进步组(P<0.01),良效和进步组与治疗无效组比较差别无显著性。(P>0.05)。结论ITP患者外周血Treg比例降低,提示Treg可能参与了ITP的发病机制。

Objective To analysis the rate of CD4^＋CD25^＋ regulatory T cell （Treg） in peripheral blood in the patients with immune thrombocytopenie purpura （ITP） and to study the role of Treg in immune thrombocytopenie purpura. Methods Peripheral blood mononuclear ceils （PBMCs） were generated from 42 ITP patients cured normally and the population of CD4^＋CD25^＋ Treg was assessed by flow cytometry subsequently. Results The proportion of CD4^＋CD25^＋-Treg in the group of ITP before therapy was increased after therapy, but significantly less than that of health control group. However the proportion of of CD4^＋CD25^＋-Treg in post-therapy group of ITP was less than that of health control group. The proportion of Treg in th.e effective therapy group was higher than that of in the refractory group. No significant difference between the preferably effective therapy group/progressing therapy group and no effective therapy group was detected. Conclusion The low percentage of CD4^＋CD25^＋-Tregs in ITP and subsequent defect in suppressing in cellular immune response may be involved in pathophysiology of ITP.