山茶属红山茶组植物的RAPD分析及分类研究

RAPD Analysis and Taxonomy of Sect. Camellia Species in Camellia

以山茶属29种红山茶组植物叶片为材料抽提DNA,利用筛选出的23种随机引物对其进行PCR扩增,将扩增出的谱带转化成0-1型数据,用UPGMA法进行相关分析和聚类分析。结果表明:可以将山茶属红山茶组的29个种划分为4大类,第1类为光果红山茶亚组,第2类为毛蕊系,第3类为滇山茶系,第2类和第3类属于滇山茶亚组,短管红山茶单独聚为一类。其结果与张宏达分类系统基本一致。根据各种之间的相容关系系数的大小,发现红山茶组内有3组亲缘关系较为相近的种。

The leaf DNA samples of 29 species of Sect. Camellia in Camellia were extracted and analyzed, using random amplified polymorphic DNA （RAPD） genetic markers. 23 selected primers were used for DNA amplification. The amplified polymorphic DNA bands were translated into 0 - 1 data, and then, correlation analysis and clustering analysis were done for processing data by using UPGMA method. The results indicated that 29 species of Section Camellia should be grouped under four clusters, that is, Subsect. Lucidissima, Series Chrysanthae and Series Reticulata, moreover, C. glabsipelata was taken as a sole cluster. Series Chrysanthae and Series Reticulata composed Subsect. Reticulata. The results were in agreement with the views of Zhang Hongda＇ s taxonomy. Based on similarily coefficient between each other, 3 varieties groups with close genetic relationship were discovered in Section Camellia.