光肩星天牛mtDNACOⅠ基因遗传差异的研究

Genetic Variation of mtDNA COⅠ in Anoplophora glabripennis(Coleoptera:Cerambycidae) Populations

应用PCR直接测序法对不同地理来源的24个光肩星天牛、4个不同地理来源的黄斑星天牛及8个近缘种样品和花柳曲窄吉丁共37个样品的线粒体mtDNACOⅠ基因中一段504bp的序列进行测序。序列分析结果表明:光肩星天牛与其近缘种、外缘种间存在明显规律性差异;中国、美国、韩国等不同地理来源的光肩星天牛样品间存在一定的基因差异,并呈现出中国北方、中国南方、美国和韩国4个种群的特征,此研究结果不支持“美国的光肩星天牛是从中国传入的”的观点;光肩星天牛和黄斑星天牛样品间的差异较小,并未达到种级分类水平,应归为同1个种。

504 bp fragment of mtDNA CO Ⅰ gene of 37 individuals including 24 geographical populations of Anoplophora glabripennis, 4 geographical populations of Anoplophora nobilis, 8 sibling species and Agrilus marcopoli Obenberger were sequenced. The result indicated that there were evidently regular differences between Anoplophora glabripernnis populations and their sibling species. Sequence of samples from China, USA and Korea had their own sequence character, and reveals four populations among those samples： north China, south China, USA and Korea. Thus there weren＇t any evidence to prove that Anoplophora glabripernnis occured in the USA were from China. Results of this study also showed that there were little different bases between Anoplophora glabripernnis and Anoplophora nobilis which strongly supports the findings that the two may in fact be a single species.