溃疡性结肠炎与结直肠癌中MUC2、p53、PCNA的表达及临床意义

Expression of MUC2,p53 and PCNA in UC and colorectal carcinoma tissue

目的:探讨溃疡性结肠炎(UC)与结直肠癌(CRC)组织中粘蛋白2(MUC2)、p53和增殖细胞核抗原(PC-NA)3种标记物的表达,为UC癌变的诊治提供依据。方法:采用免疫组织化学Evision二步法,对71例UC和59例CRC病检组织中MUC2、p53和PCNA 3种标记物的表达进行检测。结果:(1)UC、CRC组织中MUC2阳性表达率分别为97.19%、81.03%,p53阳性表达率分别为6.67%、45.76%,PCNA阳性表达率分别为74.24%、96.55%,UC和CRC组织中各标记物阳性表达率差异均有统计学意义(P<0.05)。(2)在CRC组织中,MUC2和p53的表达有一定关系,MUC2阳性表达的组织,p53多呈阴性表达;MUC2阴性表达的组织,p53多呈阳性表达(P<0.05)。(3)p53和PCNA也有一定关系,p53阳性表达的组织,PCNA多呈阳性表达;p53阴性表达的组织,PC-NA多呈阴性表达(P<0.05)。(4)MUC2与PCNA的表达无明显关系(P>0.05)。结论:MUC2表达下调和p53、PCNA高表达可能与UC癌变的发生有关。

Objective： To study the expression of mucin 2 （MUC2）, p53 and proliferating cell neuclear anti gen （PCNA） in ulcerative colitis （UC） and colorectal carcinoma （CRC） tissue, to find the evidence of colo rectal carcinogenesis from UC. Methods： Expression of MUC2, p53 and PCNA were detected by immunohistochemistry （Evision） in the tissue of 71 UC and 59 CRC specimens. Results： In the tissue of UC and CRC, the positive expression of MUC2 were 97.19%, 81.03%; p53 were 6.67%, 45.76%; PCNA were 74.24%, 96.55% respectively. These differences all had signification （P 〈0.05）. In the tissue of CRC, MUC2 had relation to p53： the tissue of positive expression of MUC2, the expression of p53 was often negative; the tissue of negative expression of MUC2, the expression of p53 was often positive （P 〈0.05）. p53 also had relation to PCNA： the tissue of positive expression of p53, the expression of PCNA was often positive; the tissue of negative expression of p53, the expression of PCNA was often negative （P 〈0.05）. MUC2 showed no relation to PCNA （P 〉0.05）. Conclusion： The down-regulaed expression of MUC2 and the up-regulated expession of p53 and PCNA may show signification in colorectal carcinogenesis from UC.