摘要
研究制作了一种可在线检测发酵过程中气相氧、溶解氧及中间补料、采样的特殊摇瓶，取得了摇瓶规模下的菌体摄氧率、氧传递系数等重要工程参数，并以摇瓶所得摄氧率（ＯＵＲ）为依据进行发酵放大，通过质谱仪在线检测，验证发酵罐发酵与摇瓶发酵所得数据的异同。
Some important ferment. eng. paramenters, such as: oxygen uptake rate and oxygen transfer coefficient, etc. , were obtained from a specially designed shake flask, in which the gasphase oxygen and liq-phase oxygen could be on-line detected in the ferment. course, based on the permeability P_m determined by the method of sulfite oxidation. Ferment. scale-up were carried on the basis of oxygen uptake rate obtained from the shake flask, through mass spectrometer on-line analysis, a comparison of ferment. parameters obtained between fermentor and shake flask was made.
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
1996年第1期91-95,共5页
Chemical Reaction Engineering and Technology
基金
国家教委博士点基金
陕西省教委专项基金
关键词
摇瓶
在线检测
溶解氧
发酵参数
气相氧
Shake flask, Ferment. eng. parameters, On-line detection, Ferment. scaleup
