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氯化硝基四氮唑蓝还原法检测血清糖化低密度脂蛋白及极低密度脂蛋白的方法学研究及应用 被引量:2

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摘要 目的建立用氯化硝基四氟唑蓝(NBT)还原的方法测定血清糖化低密度脂蛋白及极低密度脂蛋白(SCLP-VP)。方法采用在碱性条件下,SCLP-VP可使NBT还原为紫色的甲腊,其颜色的深浅与SCLP-VP的含量成正比的原理,在721型分光光度计上测定血清中的SCLP-VP。结果150例正常健康成年人SGLP-VP指数为20.20±9.13(^-x±SD);批内CV为2.96%-4.25%;批间CV为2.73%~5.96%;与空腹血糖水平比较有显著性差异(r=0.935。P〈0.005)。结论该法简便,反应迅速,重复性好,元需特殊仪器和试剂,基层实验室均可使用。
作者 王振清
出处 《卫生职业教育》 2006年第11期105-106,共2页 HEALTH VOCATIONAL EDUCATION
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