人血浆中苯巴比妥、拉莫三嗪、奥卡西平及其单羟基代谢物的HPLC测定

Determination of Phenobarbital, Lamotrigine, Oxcarbazepine and Monohydroxycarbazepine in Human Plasma by HPLC

建立了HPLC法同时测定人血浆中苯巴比妥、拉莫三嗪、奥卡西平及其代谢物单羟奥卡两平浓度。以氯唑沙宗为内标,血浆样品以蛋白沉淀法处理。采用XDB-C18柱,流动相为甲醇-0．1％三氟乙酸(38:62)。双波长双通道检测,分别在215nm测定苯巴比妥和单羟奥卡西平,240nm测定拉莫三嗪和奥卡西平。4种药物的线性范围分别是5～100、1～50、0．5～50、2～100μg／ml。平均方法回收率分别为101．3％、100.7％、96．7％和101．5％。

A HPLC method was established for the simultaneous determination of phenobarbital, lamotrigine, oxcarbazepine and monohydroxycarbazepine in human plasma. The analytes were extracted by protein precipitation, using chlorzoxazone as internal standard. A XDB-C18 column was used with the mobile phase of methanol-0.1% triflouroacetic acid （38：62）. Phenobarbital and monohydroxycarbazepine were detected at the wavelength of 215nm, while lamotrigine and oxcarbazepine at 240 nm. The calibration curves were linear in the ranges of 5 - 100, 1 - 50, 0.5 - 50 and 2 - 100μg/ml, respectively. The average recoveries were 101.3 %, 100.7 %, 96.7 % and 101.5 %, respectively.