青枯雷尔氏菌HPLC分析中色谱分离条件的研究

Study on the separating conditions of Ralstonia solanacearum by high performance ion exchange liquid chromatography analysis

研究了在青枯雷尔氏菌的高效离子交换色谱（HPLC）分析中,不同色谱分离条件对分离效果的影响.建立了在室温下分离青枯雷尔氏菌的最佳色谱条件：采用Toyopearl SuperQ-650C强阴离子交换树脂、 0.02 mol/L哌嗪-HCl缓冲液（pH 8.0）、洗脱盐浓度为1 mol/L NaCl、洗脱梯度0～75%/30 min、流速1 mL/min.在该色谱条件下,可获得良好分离的3个青枯雷尔氏菌色谱峰.

The separating effect of different chromatographic conditions of Ralstonia solanacearum by high performance ion exchange liquid chromatography analysis was studied. The optimal chromatographic conditions for separating Ralstonia solanacearum are as follow： Toyopearl SuperQ - 650C anion resin, 0.02 mol/L piperazine - hydrochloric acid buffer （pH 8.0）, elution salt 1 mol/L NaCl, elution gradient 0 - 75 %/30 min, flow rate 1 mL/min. Under these conditions, Ralstonia solanacearum is well separated into three bacterial fractions.