子宫内膜异位症患者血管内皮生长因子的检测及其临床意义

Determination of vascular endothelial growth factor in patients with endometriosis and its clinical significance

目的 旨在探讨血管内皮生长因子（VEGF）在子宫内膜异位症（EM）发病机制中的作用，并初步探讨VECG在EM中的诊断价值。方法 采用定量双抗体夹心酶联免疫吸附法（ABC-ELISA）测定44例EM患者（Ⅰ～Ⅱ期10例，Ⅲ期20例、Ⅳ期14例），20例对照组和18例术后经孕三烯酮治疗3个月的EM患者腹腔液和血清中VEGF的浓度。分析EM患者腹腔液中VEGF与EMr-AFS分期的关系，并比较VEGF在血清、腹腔液中的含量，同时利用ROC曲线初步分析其诊断EM的灵敏度和特异性。结果 ①EM患者腹腔液中VEGF浓度显著高于对照组（P〈0．05）；VEGF浓度在EM患者Ⅰ～Ⅱ期和Ⅲ期、Ⅳ期比较差异均无统计学意义（P〉0．05）；ⅡEM患者血清中VEGF浓度高于复查组，差异有统计学意义（P〈0．05）；③ROC曲线发现，EM患者腹腔液中VEGF浓度对EM的诊断具有一定价值。结论 VEGF在EM的发病机制中可能发挥着重要作用，抗血管生成治疗方法有望成为EM新的治疗策略和靶点。

Objective To determine the role of vascular endothelial growth factor （VEGF） in the pathogenesis of endometriosis （EM） and to evaluate its diagnostic values. Methods The level of VEGF in peritoneal fluid （PF） and serum was measured using enzyme linked immunosorbent assay （ELISA） from 44 women with EM （stage Ⅰ , Ⅱ n = 10,stage Ⅲ n =20,stage Ⅳ n = 14） and without EM （control group n =20） as well as progestin-treated group postoperatively for 3 months （ n = 18）. The relation of VEGF in PF to EM and r-AFS was analysed,and the VEGF concentration in PF and serum were compared. At the same time, receiver operator characteristic curve （ROC） was used to analyze the sensitivity and specificity of VEGF in PF for diagnois the EM. Results ①The VEGF level in PF of EM patients was higher compared to control group （P〈 0.05） ;②The VEGF level in serum of EM patients was significantly higher compared to counterhack group （P 〈 0.05） ; ③VEGF in PF is helpful for diagnosis the EM. Conclusion These data suggest that VEGF may play an important role in the pathogenesis ot EM. The tree of angiogenesis inhibitor may provide a novel therapeutic approach for treating the EM.