松南结缕草成熟胚愈伤组织的诱导和再生

Embryogenic callus induction and plant regeneration from mature seed of Zoysia japonica cv.Songnan

松南结缕草成熟种子经灭菌和打破休眠后，接种于含有不同浓度2，4-D的MS和N6培养基上进行愈伤组织诱导。结果表明，结缕草种子的休眠主要在于破除种壳。2，4-D浓度在2～5mg／L对愈伤组织诱导率无明显影响。基本培养基MS和N6诱导效果相近，初生愈伤组织为白色、半透明，质地松软、水浸状。继代培养基中添加一守浓度的甘露醇、麦芽糖和ABA以及延长继代时间有助于愈伤组织的胚性化。基本培养基MS更适于结缕草初生您伤组织致密胚性化。胚性愈伤组织在MS添加0．1mg／L2，4-D培养基上即可分化出大量具有根和茎叶的再生植株。

Callus induction was induced by inoculating sterilized, mature Zoysia japonica cv. Songnan seeds with broken dormancy on solidified MS and N6 media containing different concentrations of 2,4-D. The results showed that the main reason for dormancy of Z. japonica seed was the hull. There were no significant effects of concentrations of 2,4-D between 2-5 mg/L or of basal medium MS and N6 on callus induction frequency and appearance. The appearance of primary callus was white, translucent, and sticky and was accompanied by a high water content. Subculturing onto a medium supplemented with mannitol, maltose, or ABA and with prolonged subculture time promoted embryogenic callus induction. For embryogenic callus induction and maintenance, basal medium MS was better than N6. Shoots were regenerated from embryogenic callus in MS medium containing 0.1 mg/L 2,4-D.