急性不同强度至力竭运动对大鼠肝细胞凋亡的影响

Research on Effects of Acute Exerciser to Exhaust on Liver Apoptosis in Rats

研究方法:将成年雄性SD大鼠30只随机分为对照组、中等强度至力竭运动组和大强度至力竭运动组。测定肝线粒体中的Ca2+含量,观察凋亡肝细胞核,以探寻运动与肝细胞凋亡的相关性。研究结果:1)对照组的肝细胞中检测到少量TUNEL阳性细胞核,ME组、HE组出现凋亡细胞核;运动组与安静组相比,差异显著(p<0.05),并且不同强度组组间的差异显著(p<0.05);2)HE染色显示运动组肝细胞明显肿胀,肝窦变窄甚至消失,核变小,深染,肝小叶轮廓改变。3)中等强度力竭性运动后,肝线粒体钙含量比对照组增加263%,差异非常显著(p<0.01)。运动组线粒体钙含量有大量的增加,与对照组相比差异具有显著性意义,不同强度组间的差异显著(p<0.05)。结论:线粒体内Ca2+的大量积聚可能激活了细胞凋亡的启动程序,这使得中等强度力竭运动更易于导致肝细胞凋亡。

Methods Forty male adult SD rats were randomly divided into three groups： control （C）, middle ittensity exercise （ME） and high intensity exercise（HE）. The mitochondrial Ca^2＋ content were examined, the apoptotic liver cell were detected by TUNEL. Results： 1 ） Fewer TUNEL positive in the liver was detected in liver of the control group. In HE group and in ME group, there were a number of detected apoptosis cells in liver, （p 〈 0.05） ,which was significant when compared with the control group, while the deference between two exercise groups was significant （ p 〈 0.05） ; 2） In the HE stain, liver bulged in exercise group, sinus hepaticus narrowed or died away, karyon diminished and deep dyed; liver lobule of outline change;3） The mitochondrial Ca^2＋ in the liver was increased by 263% after middle intensity exercise, and the result of the comparison with control group was significant （p 〈 0.01 ）, while there were increases in the exercise groups, the deference between two exercise groups was significant（p 〈 0.05）.