PGE_2对肝病大鼠创伤弧菌攻击后血清TNF-α、IL-10水平的影响及其肾组织的保护作用

Effect of PGE_2 on kidney ultrastructure,TNF-α and IL-10 in hepatopathy rats attacked by Vibrio vulnificus

目的探讨前列腺素E2(prostagland in E2,PGE2)免疫干预对肝病大鼠创伤弧菌(Vibrio vulnificus,Vv)攻击后TNF-α、IL-10的影响以及与肾组织超微结构改变的相关性。方法正常大鼠9只和肝病大鼠36只,分别为正常大鼠Vv攻击组、肝病大鼠Vv攻击组、肝病Vv攻击后氧氟沙星药物治疗组、肝病Vv攻击PGE2氧氟沙星联合保护组和肝病大鼠NS组(每组n=9)。ELISA法测定血清TNF-α和IL-10含量。取各组大鼠肾标本电镜下观察超微结构病理改变。结果肝病大鼠较正常大鼠Vv攻击后IL-10低而TNF-α高(P<0.05);PGE2免疫干预组肾组织超微结构明显改善。结论PGE2免疫干预能上调肝病大鼠血清IL-10,抑制TNF-α的分泌,对肝病大鼠Vv攻击后肾组织具有免疫保护作用。

Objective To study the effect of prostaglandin E2 （PGE2） on kidney ultrastructure, TNF-α and IL-10 in hepatopathy rats attacked by Vibrio vulnifwus （Vv）. Methods 9 normal rats and 36 hepatopathy rats were divided into normal rats Vv attacked group, hepatopathy rats Vv attacked group, ofloxacin treatment group, combined PGE2 with ofloxacin protect group and hepatopathy rats NS group （n = 9） , the serum levels of TNF-α and IL-10 were measured by specific enzyme linked immtmo-assay （ELISA）, kidney tissues were made ulrtrathin section and examined by electron microscopy. Results Serum IL-10 level of combined PGE2 with ofloxacin protect group was significantly higher than that of ofloxacin treatment group, however, TNF-α level of PGE2 immuno-protect group were signifcantly lower than that of ofloxacin treatment group （ P 〈 0.01 ） ; kidney injury was improved significantly after treatment with PGE2. Conclusion PGE2 could not only increased IL-10 concentration in the serum but also potent inhibit from TNF-α, and kidney injury hadbeen improved significantly after treantment with PGE2