整合子在临床致感染菌基因中的分布分析

Analysis on the distribution of integron in the common clinical isolates

目的检测哈尔滨医科大学二院常见致感染菌基因中Ⅰ、Ⅱ、Ⅲ类整合子的分布情况。方法对收集的86株常见致感染菌进行药敏试验,并进行聚合酶链反应(PCR),观察扩增产物的片段长度。结果86株细菌中Ⅰ类整合子阳性有40株,占46.5%,Ⅱ类整合子阳性有7株,占8.1%,尚未发现Ⅲ类整合子。整合子阳性菌表现出对β-内酰胺类、喹诺酮类等的多重耐药。结论利用PCR能够快速检测出细菌整合子携带情况,整合子与细菌耐药性密切相关,常引起多重耐药。

Objective To exam the distribution of class 1, class 2 and class 3 integron in the isolates which was easihy infected in clinic. Methods The medicine fest was faken for collected 86 isolates, and then the lengths of the production with PCR were examined. The existence of classl ,class2 and class3 integron were determined by analyzing the amplified DNA segment. Results Among 86 strains, the classl integron positive were 40 strains （40. 5% ）, the class2 inte- gron were 7 strains （ 8% ）, and class 3 integroll was not found. The integron positive strains showed off β - lactams and Quinlolne multiple resistance. Conclusions We could examine the existence of integron in isolate rapidly with PCR. Integron was involed with antimicrobial resistance.