兔自家大肠杆菌高免卵黄抗体的研制与应用

Development and Application of Highly Immunized Yolk Antibody against Rabbit E.coli

采用灭活菌苗进行基础免疫、加强免疫,灭活油乳剂疫苗进行超强化免疫和维持免疫程序。通过微量凝集试验方法,研究了鸡抗兔大肠杆菌高免卵黄抗体的消长规律及临床应用。结果表明,超强化免疫5周后,抗体效价达到高峰,9周后抗体效价逐渐下降。同一剂量卵黄抗体效价达26时,肌注2 mL/kg体重,连用3 d对兔大肠杆菌感染的治愈率达90%。成品卵黄抗体的效价应达到26、安全性检验、无菌检验阴性方可用于临床治疗。卵黄抗体在血清中维持高浓度可持续1周,然后逐渐下降。在冷冻环境下可保存1年,其抗体效价保持在有效水平。与头孢噻肟进行的对比田间治疗试验,卵黄抗体的治愈率显著高于头孢噻肟。

Chickens were immunized with inactivated oil emulsion vaccine made with E. coli isolated from rabbit for many times and their eggs were used to prepare highly immune yolk antibody and the titer of the antibody was detected by the method of mieroglutination assay. The result showed that the antibody titer reached the peak in the 5th week after super-strengthen immunization and decreased gradually after 9th week. Yolk antibody of titer not less than 6log2 was injected into muscle with the dosage of 2 mL/kg successively in three days and get a cure rate of over 90 percent. The finished product of yolk antibody applied to clinical cure should reach to the standard of a titer of 6log2, safety and asepsis. The yolk antibody could persist high concentration in serum for one week and gradually decreased afterwards. It mainmined an effective titer in frozen condition for one year. The result also declared that the yolk antibody was more effective than cefotaxime to cure rabbit colibacillosis in clinic.