灯盏花素对鼠主动脉平滑肌钙激活钾通道的激活作用

Activation of breviscapine to calcium-activated potassium channels in rat aortic smooth muscle

目的:观察灯盏花素对大鼠主动脉平滑肌钙激活钾通道(KCa)的影响,揭示灯盏花素在分子水平的作用机制。方法:采用Wistar大鼠20只,应用膜片钳技术,研究灯盏花素对大鼠主动脉平滑肌KCa的作用。结果:细胞贴附式膜片上,应用浓度分别为5×10-4、10-3、1.85×10-3mol/L的灯盏花素后,大鼠的通道开放概率(Po)及通道电导明显增大:Po分别由用药前的0.012±0.047增加至0.427±0.364,0.609±0.312(P<0.05),0.839±0.192(P<0.01);通道电导分别由用药前的(124±59)pS增大至(174±83)pS,(199±41)pS(P<0.05),(269±85)pS(P<0.01)。结论:灯盏花素可激活KCa,从而起到舒张血管的作用。

Objective：To explore the mechanism of breviseapine at molecular level by investigating the effect of breviscapine on calcium-activated potassium channels（KCa） in rat aortic smooth muscle. Method：Wistar rats were used,and the action of breviscapine on KCa was studied in rat aortic smooth muscle using the Patch clamp tech nique. Result：In cell-attached patch,both the channel open probability（Po） and the channel conductance were enhanced by the application of breviscapine. When the concentration of breviscapine was 5 × 10^-4 , 10^-3, 1.85×10^-3mol/L, the Po was increased from （0. 012±0. 047） to （0. 427±0. 364）, P 〈0.05,0. 609±0. 312 （ P〈0.05）, 0. 839±0. 192 （ P〈0.05） respectively,and the channel conductance was increased from （124±59） pS to （174± 83）pS,（199±41）pS（P〈0.05） ,（269±85）pS（P〈0.01） respectively. Conclusion：Brcviscapine may activate KCa and play a role in dilating vasculature.