用血清药理学方法观察血府逐瘀浓缩丸对实验性动脉粥样硬化家兔主动脉平滑肌细胞增殖的影响

Effect of Concentrated Xuefu Zhuyu Pill on Proliferation of Vascular Smooth Mascle Cells in Experimental Atheriosclerosis Rabbits Observed by Serologic Pharmacological Test

采用两种离体实验方法对比观察血府逐瘀浓缩丸对实验性动脉粥样硬化家兔主动脉平滑肌细胞（ＳＭＣ）增殖的影响。第一种采用给家兔灌服血府逐瘀浓缩丸后取其血清加至细胞培养基中进行实验即血清药理学方法；第二种直接加血府逐瘀煎剂浓缩液至培养基中。结果发现含血府逐瘀浓缩丸血清和血府逐瘀煎剂浓缩液均对ＳＭＣ增殖有抑制作用，并呈剂量依赖性，且煎剂浓缩液作用强于含药血清。表明血府逐瘀浓缩丸在体内外确均有抑制ＳＭＣ增殖作用，血府逐瘀浓缩丸确含抗ＳＭＣ增殖的有效成分，并且此成分口服有效。血府逐瘀煎剂浓缩液的抑制作用比含药血清强，可能与某些有效成分未能从消化道吸收或经体内代谢失活有关。

Two in vitro experiments were performed for comparative study to evaluate the effect of concentrated Xuefu Zhuyu pill(CXZP), a Chinese traditional herbal medicinal mixture, on the proliferation of vascular smooth muscle cells(SMCs) of experimental atherosclerosis rabbits by serologic pharmacological test. The first experiment approach adopted the addition of the rabbit's serum to the culture medium while in the second experiment the CXZP was added to the medium directly. Results showed chat both the serum with CXZP and the CXZP extract itself could iuhibit the proliferation of SMC significantly and dose-dependently, and the inhibitory action of the latter was more potent than the former one. It suggested that CXZP could iuhibit the proliferation of SMC both in vivo and in vitro, the decrease of effectiveness in vivo might be due to the non-absorption of some active ingredient of CXZP or due to metabolic inactivation.