胃癌及胃粘膜非典型增生组织p21ras表达与DNA倍体关系的探讨

Relationship between Expression of p21ras and DNA Ploidy in Gastric Carcinoma and Atypical Hyperplasia

用免疫组化和自动图像分析方法检测２７例胃癌、２２例非典型增生和９例正常胃粘膜组织中ｐ２１ｒａｓ表达及ＤＮＡ倍体分布类型，发现胃粘膜非典型增生组织中ｐ２１ｒａｓ阳性率最高。２７例胃癌组织中，ＤＮＡ倍体分布类型属Ⅰ型、Ⅱ型及Ⅲ型的ｐ２１ｒａｓ阳性率分别为８３．３％、６６．７％及３３．３％。表明胃癌组织ｐ２１ｒａｓ表达水平随ＤＮＡ含量不断增多，随倍体成倍增加而逐渐下降，明显低于非典型增生组织，提示ｐ２１ｒａｓ过度表达主要出现在细胞表型发生明显转化之前。

Imunohistocbemical staining and automatic image analysis were appied to detect the expression of p21ras and DNA ploidy distribution pattern among 27 cases of gastric carcinoma,22 cases of atypical hyperplasia and 9 cases of normal mucousa.The results showed that the positive rate of p21ras was the highest in atypical hyperplasia,In 27 case of gastric carcinoma,the positive ratio of p21ras in DNA ploidy pattern typeⅠ,Ⅱand Ⅲ was 83.3%,66.7%and 33,3% respectively.It indicated that expression level of p21ras in gastic carcinoma cells decreased with increase of their DNA content and ploidy.Moreover,the expres sion level of p21ras in gastric carcinoma was obviously lower than that in atypical hyperplasia.This finding suggested that the overexpression of p21ras in gastric carcinoma might be initiated before cytophenotype transformed.