过敏性紫癜患儿血清白三烯B_4白介素-5的测定及其临床意义

Serum levels of IL-5 and LTB_4 in children with Henoch-Schonlein purpura

目的观察过敏性紫癜患儿血清白介素-5(IL-5)和白三烯B4(LTB4)在不同发病阶段的浓度变化,以探讨IL-5和LTB4在过敏性紫癜发病机制中的作用。方法采集31例过敏性紫癜患儿急性期和恢复早期血清,以及27例健康儿童血清,应用酶联免疫吸附试验检测血清中IL-5,LTB4和C反应蛋白(CRP)含量。结果过敏性紫癜患儿的急性期血清IL-5,LTB4和CRP含量明显高于恢复早期(P<0.01),恢复早期血清IL-5,LTB4和CRP含量又显著高于正常组儿童(P<0.01)。IL-5和LTB4变化与CRP呈正相关。结论过敏性紫癜患儿的急性期血清IL-5和LTB含量升高,恢复早期有所下降,提示IL-5和LTB参与了过敏性紫癜的发病过程。

Objective This study investigated the serum levels of interleukin-5 （ IL-5 ）, leukotriene B4 （ LTB4 ） and C-reactive protein （CRP） in children with Henoch-Schonlein purpura （HSP） at different phases to explore the role of IL- 5 , LTB4 and CRP in the pathogenesis of HSP. Methods Serum levels of IL-5, LTB4 and CRP in 27 normal children and 31 children with HSP at the acute phase and the early recovery phase were detected using ELISA. Results The serum levels of IL-5, LTB4 and CRP in children with HSP were 53. 8 ± 4. 2 pg/mL, 95. 3 ± 12.0 pg/mL and 36. 10 ± 11.78 mg/L, respectively at the acute phase. The values were significantly decreased at the early recovery phase （37.8 ± 3.9 pg/mL, 45.7 ± 10.1 pg/mL, 18.35 ± 6.43 mg/L; P 〈 0.01 ）, but remained higher than those in normal controls （ 12.7 ± 3.2 pg/mL, 17.6 ± 5.7 pg/mL, 4.75 ± 2.85 mg/L; P 〈 0.01 ）. The serum levels of IL-5 and LTB4 positively correlated to the CRP level. Condusions The serum levels of IL-5 and LTB4 in children with HSP increased during the acute phase and decreased at the early recovery phase, suggesting that IL-5 and LTB4 may be involved in the pathogenesis of HSP.