吡喹酮与干扰素-γ对血吸虫病小鼠肝窦病变的影响

Effect of chemotherapy and anti-fibrosis on liver sinusoid of mice infected by schistosoma japoncium

目的:观察吡喹酮和干扰素-γ(IFN-γ)对血吸虫病小鼠肝窦病变的影响。方法:将感染日本血吸虫小鼠在16周时随机分成三组:吡喹酮治疗组、吡喹酮联合IFN-γ治疗组以及模型组,治疗8周,另取10只正常小鼠为对照组。肝标本行常规病理染色,进行肝纤维化程度的半定量分析,免疫组化染色检测肝窦IV型胶原(C-IV)和第Ⅷ因子相关抗原(vWF)的表达,另每组取2例小鼠肝组织进行肝窦透射电镜的检查。结果:吡喹酮治疗组肝窦内皮细胞(SEC)未见窗孔,可见基底膜(BM),肝纤维化程度、肝窦C-IV和vWF的表达下降均不明显(P>0.05)。吡喹酮联合IFN-γ治疗组SEC可见少数窗孔,未见BM,肝纤维化程度、肝窦C-IV和vWF的表达均下降明显(P<0.05或P<0.01)。结论:IFN-γ可改善血吸虫病肝窦病变的作用,并可能据此作用实现其抗肝纤维化作用。

Objective：To observe the effect of praziquanteland and interferon-γ on liver sinusoid of mice infected by schistosoma japonicum. Methods：The mice were divided randomly into three groups after infection at 16 week：infection group, praziquantel treated alone group and the combination of praziquantel and interferon-γ treated group. The infected mice were treated for 8 weeks and 10 normal mice were as the control. The liver specimens of mice were used for pathological examination to get to know the degree of liver fibrois by semi-quantify and observe the expression of C-Ⅳ and vWF in liver sinusoid by immuhistochemisty staining. The ultramicrostructure of the liver tissue was observed by transmission electron microscope.Results：Mter chemotherapy with praziquantel, the fenestration in SEC could not be observed, the base membrane could be observed, the liver fibrosis degree, the expression of C-Ⅳ and vWF in liver sinusoid remained consisitent with infection group （P〉0.05）. When treated with praziquantel and interferon-γ the fenestration in SEC could be observed, but the base membrane could not be observed, the liver fibrosis degree, the expression of C-Ⅳ and vWF in liver sinusoid decreased obviously （P〈0.05 or P〈0.01）. Conclusion：Interferon-γ can reverse pathological changes in liver sinusoid induced by sehistosoma japoncium, and it＇s anti-fibrotie effect may depend on this.