Southern印迹技术检测人9种恶性淋巴瘤细胞系IgC_λ基因的重排

Detection of Immunoglobulin C_λ Gene Rearrangement in Nine Kinds of Malignant Lymphoma Cell Lines Using Southern Blotting Technique

用Southern印迹技术分析人9种恶性淋巴瘤细胞系,并与正常胚系DNA相比,胚系DNAIg C_2基因表现为14kb、8kb、5kb片断,而Su-DHL-2、Nu-DHL-1、Raji、SB、Daudi细胞系表现为重排,8392细胞系表现为部分缺失,仅Su-DHL-4、Su-DHL-5、PA-3表现为胚系结构.每种细胞系个体的特异性重排是其恶性克隆起源的基因标志.这对于了解B淋巴细胞肿瘤生成、发展、演变的机制.鉴别髓、淋细胞和T、B细胞的恶性起源、基因分型、判断药物敏感性、检测微小残留疾病均有重要意义.

Nine kinds of malignant lymphoma cell lines were compared with normal germline DNA using Southern blotting technique. Cλ gene which was derived from normal germline DNA showed 14 kb, 8 kb and 5 kb fragments. Meanwhile, rearrangement emerged from Su-DHL-2, Nu -DHL-1, Raji, SB and Daudi cell lines. Partial deletion appeared in 8392 cell line. Only Su-DHL-4,Su -DHL-5,PA -3 cell lines had germline configuration. The individual immunoglobulin gene rearrangement in every line provided a gene marker which originated from malignant clone. The special significance of this study contributed to understanding the machanism of growth, development and evolution in B-cell neoplasmas, differentiating myeloid and lymphoid cells and malignant origin of T, B cells, establishing gene typings, detecting drug sensitivity, and finding minimal residual disease.