摘要
目的:观察亚硒酸钠对大鼠肾小球系膜细胞系HBZY1表达p38丝裂原活化蛋白激酶(p38MAPK)和单核细胞趋化蛋白1(MCP1)的影响,从而研究硒在防治糖尿病肾病(DN)中的作用机制.方法:分别以高葡萄糖、高胰岛素、过氧化氢和糖基化终末产物(AGEs)刺激HBZY1细胞;先给予100nmol/L亚硒酸钠预处理后,再分别以上述4种刺激因素孵育HBZY1细胞,分别检测HBZY1细胞p38MAPK和MCP1的表达并比较.结果:4种刺激因素均可作为独立因素,导致HBZY1细胞p38MAPK和MCP1表达量增加;亚硒酸钠能抑制上述4种因素所致的p38MAPK和MCP1的表达.结论:亚硒酸钠通过抑制p38MAPK和MCP1在HBZY1细胞的表达,从而有效防治DN的发生发展,表明硒在DN的防治过程中发挥积极作用.
AIM： To observe the effect of sodium selenite on expressions of p38 mitogen-activated protein kinase （p38MAPK） and monocyte chemoattractant protein-1 （ MCP-1 ） in rat mesangial cell line HBZY-1, and to study the mechanism of sodium selenite in preventing diabetic nephropathy. METHODS： Cell line HBZY-1 was incubated with high glucose, high insulin, H2O2 and advanced glycosylation end products （AGEs）, respectively （control group）; simultaneously, the cell line HBZY-1 pre-treated with sodium selenite was also incubated with the above factors （experimental group）. The expressions of p38MAPK and MCP-1 were detected and compared in the 2 groups. RESULTS： Four factors increased the expressions of p38MAPK and MCP-1 indepently; sodium selenite inhibited the expressions of p38MAPK and MCP-1 by the 4 factors distinctly. CONCLUSION ：Sodium selenite can suppress the expressions of p38MAPK and MCP-1 in the cell line HBZY-1, which suggests that sodium selenite may play a significant role in the prevention of diabetic nephropathy by the inhihition of the expressions of p38MAPK and MCP-1 in the cell line HBZY-1.
出处
《第四军医大学学报》
北大核心
2006年第11期964-967,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30370670)
