中国人群瘢痕疙瘩家系易感基因的定位研究

Study on location for keloid susceptible locus in Chinese pedigrees

目的:定位中国人群瘢痕疙瘩家系的易感基因.方法:选择2个中国人群瘢痕疙瘩大家系,从中选出51名成员,采集其外周静脉血样,提取基因组DNA;参照国外最近相似研究的文献报道,采集其外周静脉血样,提取基因组DNA;参照国外最近相似研究的文献报道,在染色体2q23上选取6个微卫星标记,经PCR扩增,产物片断基因分型,再进行连锁分析.结果:在重组率θ=0时,这些微卫星标记的两点LOD值绝大部分<-2,排除连锁关系存在.结论:中国人群瘢痕疙瘩家系的易感基因位点不在染色体2q23上.

AIM： To locate keloid susceptible locus in Chinese pedigrees. METHODS： Peripheral venous blood samples from 51 members in 2 large keloid pedigrees were collected for extracting genomic DNA. Six markers on chromosome 2q23 were selected as microsatellite markers according to recent foreign literatures on the similar study. Subsequently, these markers were amplified by PCR, and all PCR products were genotyped and the linkage analyses were performed. RESULTS： Most of the twopoint LOD scores for these markers at θ = 0 were less than -2, which excluded the linkage. CONCLUSION： The keloid susceptible locus in Chinese pedigrees were not on chromosome 2q23.