部分液体通气治疗家猪急性肺损伤的抗炎效应

Anti-inflammatory effect of partial liquid ventilation on acute lung injury

目的:探讨部分液体通气治疗肺灌洗诱导的急性肺损伤家猪模型时,是否具有抗炎作用。方法:16只健康家猪,采用生理盐水肺内灌洗复制急性肺损伤模型,随机分为部分液体通气组及机械通气组给予不同治疗,观察其肺脏湿/干比值及肺通透指数,观察其血浆、支气管肺泡灌洗液及肺组织匀浆中TNF-α、MDA含量及SOD、MPO活性。结果:(1)部分液体通气组家猪肺脏湿/干比值、肺通透指数及支气管肺泡灌洗液中白细胞计数明显低于机械通气组。(2)肺组织MDA、MPO含量部分液体通气组明显低于机械通气组,但两组间SOD活性无明显差别。(3)部分液体通气组支气管肺泡灌洗液及肺组织匀浆中TNF-α含量明显低于机械通气组。结论:部分液体通气改善动物肺损伤指标,提示以氟碳化合物为呼吸媒介的部分液体通气对肺灌洗诱导的急性肺损伤家猪具有抗炎效应。

AIM： To investigate the anti - inflammatory effect of partial liquid ventilation in the piglets with lung lavage - induced acute lung injury. METHODS： 16 piglets were treated by lung lavage to a partial pressure of oxygen in arterial blood （PaO2） below 100 mmHg for one hour and randomly divided into mechanical ventilation group （MV group） and partial liquid ventilation group （PLV group）. Blood - gas analysis, hemodynamics, the contents of TNF - α, MDA, SOD, and MPO activity in lung tissue were measured. RESULTS： （ 1 ） Lung W/D, PPI and the count of WBC in the BALF in PLV group were significantly lower than those in MV group. The contents of MDA and MPO in lung tissue in PLV group were significantly lower than those in MV group. However, no evident difference in SOD activity in lung tissue between two groups was observed. （2） The contents of TNF -α in lung tissue in PLV group were significantly lower than those in MV group. CONCLUSION： Partial liquid ventilation with perfluorocarbon has anti - inflammatory effect on lung lavage- induced acute lung injury in the piglets.