化瘀消瘤方含药血清对体外培养的人肺癌细胞周期的影响

Effect of huayu xiaoliu fang on cell cycle of a human lung carcinoma cell line

目的:探讨化瘀消瘤方含药血清对肺癌细胞周期的作用。方法:制备高、中、低剂量含药血清、西药血清和对照血清,作用于人肺癌细胞。MTT法观察血清对细胞增殖的作用,流式细胞仪进行细胞周期时相分析。结果:高剂量含药血清组与对照血清组之间的吸光度差异有统计学意义(P<0·01),中剂量血清组、高剂量血清组的S期细胞所占比例与对照血清组之间的差异有统计学意义(中剂量血清组P<0·05,高剂量血清组P<0·01)。结论:本实验提示化瘀消瘤方含药血清可能是通过干扰细胞DNA合成而抑制肺癌细胞的增殖,为该药的临床应用提供了科学的实验依据。

AIM： To investigate the effect of huayu xiaoliu fang, a Chinese medicine, on the cell cycle of human lung carcinoma cell line by serologic pharmacological method. METHODS：PGLH7 cells were incubated with rabbit serum containing huayu xiaoliu fang at different doses obtained by serologic pharmacological method. MTT assay was used to calculate the proliferation inhibition rote. The target cells were harvested to analyze the cell cycles by flow cytometry. RESULTS：The Chinese medicine- containing senan inhibited the growth of PGLH7 cells significantly. There was remarkable difference,in the proliferation inhibition rate between 10% （high dose） Chinese medicine - containing serum and the control serum （ P 〈 0.06）. Cell cycle analysis in PGLH7 cells showed that there was significant difference in S phase between 10% high or medium dose Chinese medicine - containing serum and the control serum by flow cytometry （medium dose Chinese medicine - containing serum P 〈 0.06, high dose Chinese medicine - containing serum P 〈 0.01 ）. CONCLUSION： The antineoplastic mechanisms of huayu xiaoliu fang may be due to inhibiting DNA synthesis to restrain PGLH7 cells proliferation. It may provide a theoretical basis for the Chinese drug to popularize and apply in clinical practice.