鲁斯可皂苷元对HL-60与EC V304细胞黏附的影响

Effects of ruscogenin on adhesion of HL-60 cells to ECV304 cells

目的考察麦冬中主要皂苷元鲁斯可皂苷元(Rusco-gen in)抑制细胞黏附的作用,为深入研究其抗炎作用机制提供依据。方法采用MTT比色法检测Ruscogen in对人原髓性白血病细胞株HL-60细胞与正常或肿瘤坏死因子-α(TNF-α)活化的人脐静脉内皮细胞株ECV304细胞黏附的影响及其对ECV304与HL-60细胞增殖的影响。结果Ruscogen in 0.1,1.0μmol.L-1预处理ECV304细胞后,均抑制TNF-α诱导的ECV304细胞与HL-60细胞黏附的增加,Ruscogen in 0.001,0.01,0.1μmol.L-1预处理HL-60后,亦抑制TNF-α诱导的ECV304细胞与HL-60细胞黏附的增加;同时Ruscogen in在实验浓度范围内不影响ECV304细胞与HL-60细胞的增殖及二者之间的正常黏附。结论Ruscoge-n in可通过抑制HL-60细胞与活化的ECV304细胞之间的黏附作用,发挥抗炎活性。

Aim To investigate the anti-inflammatory activity of nmscogenin, a major sapogenin from Ophiopogon japonicus, effect of ruscogenin on cell adhesion is studied. Methods By MTT assay, effect of ruscogenin on normal and TNF-α-induced adhesion of human pro-myelocytic leukemia cell strain（ HL-60 ）to venous endothelial cell strain（ ECV304 ） was observed and effect of ruscogenin on the viability of ECV304 cells and HL-60 cells were investigated. Results Adhesion between HL-60 cells and ECV304 cells induced by TNF-α was significantly inhibited when ECV304 cells were pretreated with ruscogenin at the concentration of 0. 1,1.0 μmol . L^-1. Meanwhile, ruscogenin remarkably inhibited TNF-a-induced adhesion when HL-60 cells were pretreated with ruscogenin at the concentration of 0.001, 0.01, 0.1 μmol . L^-1. On the other hand, their viability and adhesion of HL-60 cells to normal ECV304 cells were not affected at above concentrations. Conclusion Ruscogenin shows significant anti-inflammatory activity via inhibition of adherence of HL-60 cells to activated ECV304 cells.