侧脑室注射组胺对大鼠哮喘样反应可能有调节作用

Potential regulatory effects of histamine by intracerebroventricularly administration on asthma-like reaction in rats

目的观察组胺侧脑室注射对抗原攻击致敏大鼠引起的支气管收缩反应及气道炎症的影响。方法致敏大鼠侧脑室注射组胺后测定抗原激发前后的气道阻力和肺动态顺应性。计数支气管肺泡灌洗液(BALF)中白细胞总数和分类计数。病理切片观察肺组织炎症细胞浸润情况。结果组胺(0.1μg.只-1)侧脑室注射明显抑制抗原攻击致敏大鼠所致的气道阻力升高和肺动态顺应性的降低,也明显降低BALF内的白细胞总数、嗜酸性粒细胞和中性粒细胞的数量(P<0.01,P<0.01),减少气道粘膜下和血管周围的嗜酸性粒细胞浸润。结论组胺侧脑室注射能抑制大鼠支气管收缩反应及气道炎症,提示中枢组胺水平升高可能对大鼠哮喘样反应有调节作用。

Aim To investigate regulation effects of histamine （ 0.1 μg, icy ） on bronchoconstriction and airway inflammation induced by antigen in sensitized rats. Methods Histamine were administrated intrace.rebroventricularly in sensitized rats, the airway resistance （ RL ） and dynamic lung compliance （Cdyn） before and alter antigen-challenged were observed. The total white cell and differential cell count in Bronchoalveolar lavage fluid （ BALF ） were performed. Inflammatory cells infiltration were determined by lung pathological section. Results Histamine （0. 1 μg, icv） inhibited the increase in RL and the decrease in Cdyn induced by antigen challenge, significantly reduced total white cell counts and the number of eosinophil and neutrophil （P〈0.01, P 〈 0.01 respectively）. Histamine also inhibited eosinophil infiltration below airway mucosa and around blood vessel. Conclusion Histamine（icv） inhibits bronchoconstriction and airway inflammation,suggesting that the increased histamine in central nervous system may have regulatory effects on asthma-like reaction in rats.