DOI诱导的大鼠头部抽动模型脑内多巴胺转运体的研究

STUDY OF DOPAMINE TRANSPORTER IN THE RAT BRAIN OF DOI-INDUCED HEAD TWITCH RESPONSE

目的研究DOI诱导的大鼠头部抽动模型脑内多巴胺转运体改变,探讨Tourette综合征发病与突触前多巴胺神经纤维支配的关系。方法将30只Wistar大鼠随机分为模型组和对照组,应用DOI诱导大鼠头部抽动作为Tourette综合征动物模型。两组以99mTc-TRODAT-l作为配体,采用放射自显影和γ测量方法观察大鼠脑内多巴胺转运体分布及其密度。结果放射自显影结果表明模型组大鼠脑内银颗粒密度显著高于对照组,尤其是纹状体。γ测量结果显示,模型组纹状体每克脑组织含放射性占注射剂量的百分比(%ID/g)与对照组相比显著增高(P<0.01),模型组海马和大脑皮层与对照组相比有统计学差异(P<0.05)。结论DOI诱导的大鼠头部抽动模型脑内多巴胺转运体密度较正常大鼠显著增加,Tourette综合征发病与突触前多巴胺神经纤维支配增加密切相关。

Objective To study the level of dopamine transporter in the rat brain of DOI-induced head twitch response, and to evaluate the relation between the pathogenesis of Tourette syndrome and the innervation of dopaminergic presynaptic nerve fibers. Methods 30 Wistar rats were randomly divided into the model group and the control group. DOI-induced head twitch response was taken as the Tourette syndrome model. Distribution and density of DAT in rat brain were evaluated by autoradiography and γ-counting with ^99mTc TRODAT-1. Results The density of silver granules of the model group was higher than that of the control group, especially in the striatum. T-counting showed that the percentage dose/g in the striatum of the model group was significantly increased when compared with that of the control group （P〈 0. 01）, and there were significant differences in hippocampus and cortex between the two groups （P〈 0. 05）. Conclusion Compared with that of the control group, the level of dopamine transporter in the rat brain of DOI-induced head twitch response increases, and the pathogenesis of Tourette syndrome is closely related to the innervation of dopaminergic presynaptic nerve fibers.